Acta Scientiarum Polonorum

Scientific paper founded in 2001 year by Polish agricultural universities

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Medicina Veterinaria
(Weterynaria) 4 (1) 2005
Title
THE TRANSPORT AND ADAPTIVE STRESS AND WHITE BLOOD CELLS PICTURE AT CALVES
Autor
Krystyna Pogoda-Sewerniak, Witold Janeczek, Robert Kupczyński, Mariusz Korczyński
Keywords
calves, stress, transport, cortisol, glucose, leukocytes, lymphocytes CD2, CD4, CD8, B
Abstract
Research was executed on three weeks old calves, witch were transported for 45 minutes at the distance of 10 km. The calves’ blood was collected in 6 series: just before and immediately after the transport and then in 24th and 72nd hour and later: 7th, 14th, 21st day after the animals ware located in calf shed. The number of leucocytes was determined in whole blood, the smear was done, and lymphocytes were differentiated on: T, B, CD4 and CD8 cells with the immunofluorescence method including monoclonal antibodies. The concentration of cortisol was determined with RIA methods, while the concentration of glucose was detected with oxidate method. The significant increase of cortisol and glucose concentration was noticed and marked decrease in the amount of lymphocytes T and B in peripheral blood was detected directly after the transport. The increase of T lymphocytes concentration was observed 72nd hours, and B cells 14 days after the transport. In the 24th hours after of transportation the higher concentration of lymphocytes CD4 and lower concentration of CD8 cells was noticed.
Pages
37-48
Cite
Pogoda-Sewerniak, K., Janeczek, W., Kupczyński, R., Korczyński, M. (2005). THE TRANSPORT AND ADAPTIVE STRESS AND WHITE BLOOD CELLS PICTURE AT CALVES. Acta Sci. Pol. Med. Vet., 4(1), 37-48.
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