Milk samples of 65 cows of Red-and-White breed from the mountain farm (altitude 650 m) were fractionated as from the morning and evening milking for analysis. Bacteria and somatic cells counts as well as fat content, protein content and urea level differed significantly. Higher values for bacteria count (+ 56,1 thousands CFUs), SCC (+ 76 thousands), fat (+ 0,10%), protein (+ 0,11%) and urea (+ 21,6 mg/l) were stated in the milk from the evening milking. Milk urea content was positively related to fat content (r = + 0,141). Urea content as well as fat and protein contents increased in consecutive stages of lactation what was confirmed with significant correlations (consequently: r = + 0,284; + 0,183; + 0,421). It was confirmed that conversion SCC into somatic cells count score (SCCS) was favourable since coefficient of variation of this index was more resembling normal distribution (v% = 55) while SCC itself characterised with very high variability (v% = 176). Evaluation of the average level of SCC in the entire herd was in agreement with the real quality of milk produced (average: SCCS = 3,95 +/- 2,16, what is the equivalent to ca 200 thousands of somatic cells).

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