The aim of the present study was to determine the effect of medium components and culture conditions on the activity of alanyl and phenyloanalyl aminopeptidases synthesised by Pseudomonas sp. It was observed that the enzymatic activity is dependent on the composition of the culture medium. The high level of the aminopeptidase activity was achieved on the second day of growth at pH 6.8 at 27 ˚C in the mineral medium enriched with 1% of tryptone. The enrichment of the culture medium with some carbohydrates and mineral salts resulted in the increase of the activity level for both enzymes. The presence of 4% glucose in the medium resulted in a fall of the phenyloanalylaminopeptidases’ activity. In the medium with inorganic carbon and nitrogen, relatively low enzymatic activity was observed.

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