In this paper we focus on the capability to biosynthesis extra and intracellular lipases by 8 strains of Geotrichum candidum. We value activities against two different substrates: olive oil (JL_O) and glycerol tributyrate (JL_T). As a carbon source in the medium we used rapeseed oil, glucose, and residue oil – waste from fatty industry (1% of each). All tested strains show differentiation in the volume of intra and extracellular lipase activity, according to carbon source in medium, but also kind of substrate in enzymatic tests and also incubation time. Rapeseed oil was the most effective carbon source in lipase synthesis and Geotrichum candidum X5 strain showed the highest extracellular activity of lipase (260 JL_OmL^-1) in given conditions.

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