The aim of the work was to examine ability of Y. lipolytica A-101 yeast to accumulation of selenium and chromium in biomass during biomass production process in media containing crude rapeseed oil. In shaking cultures limiting growth of Y. lipolytica A-101 yeast concentration of selenium 6 mgdm^-3 Se (IV) and chromium 80 mgdm^-3 Cr (III) was obtained. Yeast of Y. lipolytica A-101 was cultivated in batch culture in stirred tank reactor, containing chemically defined medium, supplemented with selenium or chromium. The results showed that level of selenium or chromium accumulation was dependent on microelements concentration. It was found that elevated concentrations of Se (IV) and Cr (III) in the environment caused a reduction both biomass production, productivity and biomass yield of Y. lipolytica A-101. Concentrations 4 mgdm^-3 Se (IV) and 40 mgdm^-3 Cr (III) was obtained as the best concentrations for Y. lipolytica A-101 biomass production on lipid media.

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