Lactation in sheep is a high-risk period associated with hepatic metabolic disturbances and oxidative stress. The aim of this study was to evaluate oxidative status in health and having prolapsed vagina sheep during lactation, using plasma malondialdehyd and ubiquinone Q10 concentrations. The next goal was to determine hepatic functional parameters during intensive hepatic metabolism in lactating sheep. The experiments were performed on sheep, divided into two major groups: I-control-health animals and II-experimental with diagnosed prolapsed vagina Within these groups, sheep with single lamb and twins were separated. Plasma Q10 level and the profile and quantity of bile salts was analysed with HPLC method. Plasma concentration of MDA was measured by spectrophotometric method. The total glucose plasma level was determined in duplicate by the enzymatic method using a diagnostic KIT. Highest plasma concentration of MDA (2,56±0,66 nM/ml) in sheep with PV having twins was observed. In comparison to health animals, postpartum disturbances in sheep with one lamb resulted in marked (p≤0,05) decrease of Q10 concentration. In such condition plasma Q10 averaged 631,01±154,6 ng/ml; 719,47±34,78 ng/ml; 644,72±53,36ng/ml in first, second and third blood collecting period respectively. Opposite results were observed in group of sheep having twins. In 17-th day after parturition in all groups of animals plasma glucose level significantly decreased and reached minimum value (2,08±0,32mM/l) in HT sheep. The level of bile acids averaged 107,94±22,06 μM/l in HT group and 141,79±16,52μM/l in PVT group. Our data obtained in the present study suggest that disturbances occurring in lac-tating sheep directly influenced on hepatic activity and imbalanced oxidative status as well.

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