Title
DETERMINATION OF TOCOPHEROLS CONTENT IN SUNFLOWER OIL DURING OXIDATION USING FLUORESCENCE TECHNIQUE
Autor
Paweł Górnaś, Aleksander Sieger, Krzysztof Dwiecki, Małgorzata Nogala-Kałucka, Krzysztof Polewski
Keywords
sunflower oil, tocopherols, peroxide value, DPPC membrane, fluorescence
Abstract
The fluorescence intensity of tocopherols originated from cold-pressed sunflower oil at different degree of oxidation in presence of 1,2-dipalmitylo-sn-glicero-3- -fosfatidylocholine lipid (DPPC) membrane have been measured. It has been shown that fluorescence of tocopherol depends on amount of oil as well as on membrane concentration in the sample. Addition of oil with increasing peroxide value PV decreased the tocopherol fluorescence due to its disappearance in the sample. At constant membrane concentration chromatographically determined the amount of tocopherol in oil sample correlated with peroxide value and fluorescence intensity. Such results allowed us construct calibration curve which may be used for fast and accurate peroxide value determination using fluorescence technique.
Pages
157-164
Cite
Górnaś, P., Sieger, A., Dwiecki, K., Nogala-Kałucka, M., Polewski, K. (2006). DETERMINATION OF TOCOPHEROLS CONTENT IN SUNFLOWER OIL DURING OXIDATION USING FLUORESCENCE TECHNIQUE. Acta Sci. Pol. Technol. Aliment., 5(2), 157-164.
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