In this paper eight strains of Geotrichum candidum were examined for their ability to produce lipases in mineral medium containing different concentration of residues oil – waste product from fatty industry, as a carbone source. Residue oil was used in 1%, 2%, 3% concentration. Mineral medium with addition of 1% of glucose or 1% rapeseed oil was used for comparison. Activities of lipases were tested against olive oil (JL_O) and glycerol tributyrate (JL_T) used as substrates. All tested strains differed in intra- and extracellular lipase activity, depending on the concentration of oil residue as well as incubation time and the substrate type used in enzymatic analysis. Generally, enzymes of tested strains showed higher specifity to olive oil, then to glycerol tributyrate. The highest production of lipases appeared as early as the 3-th day of incubation. Higher level of lipases in presence of residue oil or rapeseed oil in comparison with control medium (containing 1% glucose as carbon source) indicates inductive character of their biosynthesis. Culture medium containing 1% of residue oil appeared to be the most effective for enzyme synthesis and G. candidum X5 strain showed the highest extracellular activity (195 JL_O mL^-1) in given conditions.

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