The aim of this work was to determine the stability of crude and pure preparations of Carnobacterium divergens AS7 bacteriocin (divercin) during long-term storage at 4 and 20 ⁰C. The influence of freezing and freeze drying on divercin activity and stability during storage were also studied. Divercin antilisterial activity was decreased during storage. The highest bacteriocin stability was obtained at 4 ⁰C in crude preparations, while the lowest activities was observed during storage pure divercin at 20 ⁰C. Freezing and freeze drying had negative effect on divercin activity in purified preparations, but the activity of crude preparations of divercin was stable during these processes. Pure divercin was also sensitive to multiple freezing. Divercin stability during long-term storage was increased after freezing and freeze drying. Antilisterial activity of divercin crude preparations were remained stable during storage at -20 ⁰C for 6 months. The same stability had also freeze dried crude preparations storaged at 4 ⁰C. Activity of pure divercin after freezing and freeze drying were decreased during storage, but divercin was more stable in freeze dried products than in frozen poducts. The divercin activity in freeze dried crude preparations increased during storage at 20 ⁰C.

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