Specific growth rate of five Yarrowia lipolytica strains growing in acetate medium (0,6%) supplemented with glycerol, ethanol or glucose (concentration range between 0 to 1%) was estimated. The studied strains differ in acetate utilisation, citric acid secretion and colony morphology. The rate of yeast growth, as well as, the biomass level changed as a consequence of the presence of second carbon source in acetate culture. Addition of ethanol inhibited (completely or partly) the growth in acetate medium of all tested strains, while glycerol and glucose stimulates the wild strain growth (A101), induced acetate growth of acu^- mutants (A101.1.31 and A101.1.31.K1) and inhibited the growth of revertant strain (A101.1.22). The maximal specific growth rate was observed for glycerol growing yeast, and a periodic variation of μ was observed in the case of very small amount of available C source. The maximal specific growth rate of wild strains on glucose was higher than on acetate.

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