Acta Scientiarum Polonorum

Scientific paper founded in 2001 year by Polish agricultural universities

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Biotechnologia
(Biotechnologia) 6 (2) 2007
Title
BIOSYNTHESIS OF PHYTASES, PHOSPHATASES, CELULASES AND XYLANASES BY FILAMENTOUS FUNGI IN SOLID STATE FERMENTATION
Autor
Anita Rywińska, Danuta Witkowska, Michał Piegza, Małgorzata Jarosz, Justyna Salamon
Keywords
filamentous fungi, phytases, phosphatases, celulases, xylanases, solid state fermentation
Abstract
In this paper we examined four strains of filamentous fungi Aspergillus niger 551, Aspergillus cervinus 219, Rhizopus nigricans 500 and Trichoderma reesei M7-1 for their ability to biosynthesis phytases, phosphatases and accompanying celulase and xylanase enzymes in solid state fermentation. As a carbon source beet pulp, wheat brain and milled bean were used. The time of the culture was 14 days and constant humidity was kept on the 63% level. All tested strains were able to synthesize dephosphorylating enzymes, celulase and xylanase depending on the strain, carbon source and incubation time. The most effective producer of phytase as well as phosphatase was A. niger 551. The medium with beet pulp was the best to produce these enzymes, the highest activity were for phytase 15,20 U g-1 (A. niger 551 – 3 day) and for phosphatase39,35 U g-1 (T. reesei M7-1 – 12 day) in these conditions. Wheat brain was the most effective carbon source for xylanase and celulase production. The highest biosynthesis of xylanase and celulase was observed for R. nigricans 500 strain, 279,5 U g-1 (3 day) and T. reesei M7-1, 12,9 U g-1 (5 day).
Pages
13-23
Cite
Rywińska, A., Witkowska, D., Piegza, M., Jarosz, M., Salamon, J. (2007). BIOSYNTHESIS OF PHYTASES, PHOSPHATASES, CELULASES AND XYLANASES BY FILAMENTOUS FUNGI IN SOLID STATE FERMENTATION. Acta Sci. Pol. Biotechnol., 6(2), 13-23.
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