The aim of the study was to examine the influence of water soluble vitamins such as: thiamin, ribolflavin, pyridoxine, vitamin C, folic acid, panthotenic acid, nicotine acid, biotin and cholin on the level of amino acids in wall intestine, liver and blood serum in chickens. Tests were done on 160 alive broiler chicken, breed Hybro G (age 2-4 months). The absorption of amino acids was determined by the method of perfused loops of small intestine. Saline including amino acids such as: L-threonine, L-valine, L-methionine, L-lysine, L-histidine, L-phenylalanine, L-leucine, L-isoleucine and water soluble vitamins in dose 100mg/l was passed through the loops. Next, level of amino acids was measured by the method of liquid chromatography. The experiment demonstrated, that water soluble vitamins undoubtedly influenced exogenous amino acid on absorption from entrails in chickens. In the wall intestine the highest level was observed after application vitamin B₆, the least reaction was after biotin, folic acid and panthotenic acid. All amino acids reacted on application vitamins in the liver. In the blood serum was observed high value amino acids after application vitamins use together and after vitamin B₆, panthotenic acid and biotin.

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