Grass pea seeds (Lathyrus sativus L.) can be an alternative protein source for calf feeding, however the content of antinutritional factors (ANFs) limits their use in the raw form, because they decrease enzymatic digestion in the intestine. The aim of this study was to determine the effect of feeding row (RGPSM) and extruded (EGPSM) grass pea seeds meal on pancreatic juice (PJ) secretion in the calf. Experiments were conducted on 6 calves that were fitted with a pancreatic duct catheter and a duodenal cannula. During 2 weeks calves were fed milk and a semiliquid diet consisting of a mixture of milk and standard formula CL (control group), RGPSM formula (row grass pea) and EGPSM formula (extruded grass pea). PJ volume, protein content, proteolytic enzymes and amylase activity were analyzed. Feeding calves with the RGPSM formula increased the PJ volume, protein content, proteolytic enzymes and amylase activity, thus feeding the EGPSM formula increased the amount of proteolytic enzymes, other than trypsin. This suggests the inactivation of the majority of ANFs during the extrusion process and the lack of negative effects on the pancreas after EGPSM formula feeding in the calf.

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