Title
THE INFLUENCE OF PRESERVATION METHOD ON CELL VIABILITY AND STABILITY OF BIOTECHNOLOGICAL CHARACTERISTICS OF YARROWIA LIPOLYTICA YEAST
Autor
Anita Rywińska
Keywords
Yarrowia lipolytica, freeze-drying, subculturing, survival, stability of characteristics, biosynthesis of citric acid
Abstract
Viability and preservation of biotechnological characteristics by citric acid producer Y. lipolytica A-101-1-31 strain during long term storage were studied. There was proved that yeasts can be preserved on agar slants under parafine layer without subculturing for two years or in freeze-dried form with milk as a protecting agent with good viability level and saving initial acidifing activity and oct- mutation that guarantee high purity of fermentation level. DMSO and its mixture with milk appeared to be not proper cryoprotectants for Yarrowia lipolytica A-101-1-31 strain liophilization.
Pages
69-80
Cite
Rywińska, A. (2002). THE INFLUENCE OF PRESERVATION METHOD ON CELL VIABILITY AND STABILITY OF BIOTECHNOLOGICAL CHARACTERISTICS OF YARROWIA LIPOLYTICA YEAST. Acta Sci. Pol. Biotechnol., 1(1-2), 69-80.
Full text