Four hundred milk samples were collected from cows. In 78 samples (19.50%) an increase of somatic cells was detected employing the California Mastitis Test (CMT). Therefore, those milk samples were plated (0.1 mL) onto bacteriological and mycological media. Cultures were incubated at 37°C and 20°C for 24h to 72h. Biochemical features of bacteria and fungi were determined in API tests and/or on liquid O-F Medium. The presence of coagulase in Staphylococcus spp. was investigated by tube test using rabbit plasma. For identification of C. albicans the germ tubes test was conducted. Based on microbiological investigation and increase of somatic cells, mastitis was confirmed in all cows, whose milk was selected for further research. The presence of bacteria was confirmed in 64.10% of samples, bacteria and yeast in 34.62% and yeast alone in 1.28%. Sixteen bacterial species were isolated: Staphylococcus spp. (5 species), Enterobacteriaceae (5 species), Streptococcus spp. (4 species), Bacillus cereus and Pseudomonas aeruginosa.Among yeasts, 15 species were identified: Candida spp. (9 species), Blastoschizomyces capitatum, Dipodascus ingens, Geotrichum candidum, Trichosporon asahii, Saccharomyces cerevisiae, Torulopsis conglobata. The most frequent isolated yeasts were Candida spp., mainly C. guilliermondii (6 strains), C. kefyr (4 strains) and C. butyri (4 strains). The most frequent isolated bacteria were Staphylococcus spp. (37 strains), Streptococcus spp. (28 strains) and Enterobacteriaceae (23 strains).

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