Abstract: An utilization of the oily waste from the food and automotive industry is both one of the most important and hard challenge for the environment protection. In this work the attempt to select the microorganisms able to biological assimilation of atypical carbon source has been introduced. The development of prowess enzymatic abilities in these microorganisms can be induced by stressogenic environment of live and enables the utilization of harmful wastes. To isolate the microorganisms a special niche was chosen, into which few different, strong detergents that inhibits the growth of microorganisms were applied. As a result of the multiphase isolation 22 strains were obtained, 18 yeasts from Candida, Trichosporon, Geotrichum and Pichia type, and 4 bacterias. Every of the microorganisms surveyed had a high ability to the oily waste assimilation. A culture with a complex carbon source, like oils from the food and automotive industry, gasoline and air-fuel was made. In the result high biomass growth was observed. Selected activities of the hydrolase were evaluated and they showed high enzymatic abilities of the tested isolates. As a model organisms, which the abilities of growth on the hard – degradation compounds were compared with, microorganisms with utilization of petroleum derivatives abilities were chosen : Yarrowia lipolytica and high-capable of lipid-degradation Geotrichum candidum.

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