Crude glycerol from biodiesel industry, containing 350 g∙dm^-3 of glycerol, was used as a substrate for citric acid production by acetate negative mutant of Yarrowia lipolytica Wratislavia AWG7 in fed-batch experiment. This strain produced 133,4 g∙dm^-3 of citric acid and 2,7 g∙dm^-3 of isocitric acid, unwanted product in this process, in 140 h of cultivation. As a results the volumetric citric acid production rate and the citric acid yield reached 0,95 g∙dm^-3h^-1 and 0,67 g∙g^-1, respectively. The total amount of other organic acids such as malic, fumaric and α-ketoglutaric acid produced by the Wratislavia AWG7 strain no exceed 6 g∙dm^-3.Polioles such as erythritol and as well as mannitol were produced in this process also. Concentration of these by-products at the and of process were 9,8 i 2,7 g∙dm^-3, respectively.

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