Simultaneous saccharification and ethanol fermentation of corn starch using enzyme preparation Stargen 001 was investigated. The experiments were conducted in an agro-distillery plant. The preparation of corn mashed based on the use of corn flour suspended in recycled liquid fraction of stillage and distillation condensates. It was shown that the final concentrations of ethanol after fermentation of 25% w/v mashes were of 8–10% w/v. The elimination of cooking for starch liquefaction allowed to reduce of energy requirements, nevertheless, made s serious problem with bacterial contamination of mashes. The concentration of lactic acid at the end of fermentation process reached up to 3.8% w/v. The SFF process connected with repeated stillage recycling allowed to remove water requirements and to perform this process as discard-less.

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