The aim of the study was to evaluate the growth of potential heterologous proteins producers: Suc⁺ clones of Yarrowia lipolytica A-101. Growth of 26 transformants, parental strain (wild) and reference strain W29ura3-302 was studied. Transformants showed differences in the ability of growth on sucrose (biomass, lag time duration, beginning of stationary phase). Beside the type of transformation, no differences in yeasts growth were observed on glucose and fructose. Influence of pH (6,8) and peptone addition (0,01 and 1%) on XPR2 promoter induction was analyzed. Both inducers were effective, allowing yeasts growth on sucrose. Growth of two transformants: KLON1 and KLON10 on sucrose was abundant, even with minimal peptone dose (0,01%). Stabilization of pH at 6,8 in the first hours of yeasts growth had a positive effect on culture. Clones grew better with buffer in the medium with 30 % sucrose, a substrate concentration which has induced longer lag phase.

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