Title
LIPASES PRODUCE BY YEAST YARROWIA LIPOLYTICA
Autor
Jolanta Krzyczkowska, Izabela Stolarzewicz, Dorota Nowak, Ewa Białecka-Florjańczyk
Keywords
lipase, lipolytic activity, extra- and intracellular enzymes, Yarrowia lipolytica
Abstract
The aim of this investigation was to report the lipase location in yeast Yarrowia lipolytica KKP 379 and relationship extracellular lipase activity with cell growth. During yeast culture in bioreactor, optical density (OD) and enzymatic activity of supernatant were measured. Intracellular lipase activity was determined before and after cell wall damage. Comparison and details for Yarrowia lipolytica KKP 379 lipase activity was determined by the chromatographic method, using ethyl oleate as substrate. The results showed that the yeast Yarrowia lipolytica KKP 379 produce extra- and intracellular lipase. Extracellular lipase activity is cell growth dependent.
Pages
25-32
Cite
Krzyczkowska, J., Stolarzewicz, I., Nowak, D., Białecka-Florjańczyk, E. (2010). LIPASES PRODUCE BY YEAST YARROWIA LIPOLYTICA . Acta Sci. Pol. Biotechnol., 9(1), 25-32.
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