In the intensive tomato production under covers, the soilless cultivation dominates. The objective of this study was to assess some quality traits of cherry tomato and middle fruit size tomato, concerning their chemical composition and sensory attributes as well as their correlation to growing medium and harvest time. Cherry tomato (Dasher F₁ and Organza F₁) and middle fruit size tomato (Admiro F₁ and DRW 7594 F₁) cultivars were grown in the years 2008–2009 in the greenhouse with controlled climate and drip fertilizing system, in three different growing media. Organic media – coconut fiber and wood fiber were compared to rockwool, commonly used as a standard horticultural medium. The quality traits that mostly differentiated tomato fruits were the cultivar, harvest time and to the lesser degree the medium quality. Tomatoes harvested in July had a higher sugar content and received a higher sensory evaluation than those harvested in September. The sugars to acids ratio was the highest in fruits grown on coconut fiber. These fruits also obtained higher notes of particular determinants of sensory analysis, especially tomato flavor. The highest quality in respect to the content of the investigated chemical components and sensory evaluation was observed in fruits of Dasher F₁ and Admiro F₁ cultivars. A significant correlation between the content of chemical quality attributes of tomato fruit with such determinants of sensory evaluation as skin firmness, tomato and sweet taste was revealed. Sugar in fruits was positively correlated with the overall tomato assessment. Firmness and sweet taste were significantly correlated with organic acids and soluble solids contents.

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