The purpose of this paper was determination of the effect of meat and bone meal applied without and with effective microorganisms compared with manure fertilization, mineral fertilizers or lack of fertilization on grain true protein content and composition of spring wheat cv. Nawra and Tybalt. The five-year studies (2005-2009) with crop rotation were conducted in Bałcyny at the Experimental and Plant Production Station near Ostróda. It was observed, that the applied fertilization with NPK as well as with manure and meat and bone meal in doses from 1.0 to 2.5 t·ha^-1 affected the significant increase in protein content in grain of the studied spring wheat cv. Tybalt in 2009. Under the effect of the applied meat and bone meal, mainly the gliadin and glutenin content in spring wheat grain of both cultivars increased. The most beneficial influence on protein composition (high percentage participation of constitutional and storage proteins in relation to the total protein content) had fertilization with 1.5 t·ha^-1 dose of meat and bone meal.

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