Title
INCREASE THE ABILITY OF BIOSYNTHESIS OF DIHYDROXYACETONE BY GLUCONACETOBACTER XYLINUS BY UV MUTAGENESIS
Autor
Stanisław Błażejak, Lidia Stasiak-Różańska, Krzysztof Markowski, Edyta Lipińska
Keywords
Gluconacetobacter xylinus, mutagenesis, UV radiation, dihydroxyacetone
Abstract
UV mutagenesis was used to obtain Gluconacetobacter xylinus mutants. The aim of this study was to attain a mutant able to produce high concentration of dihydroxyacetone. Exposure times were set to: 30, 45, 60 and 90 seconds. Mutants were isolated. The biotransformation of glycerol to DHA with use of these mutants was detected spectrophotometrically at 550 nm. The most active mutants was synthesizing 18,00 mg DHA ⋅ cm-3, what was 32% DHA ⋅ cm-3 more than the wiled type strain.
Pages
17-24
Cite
Błażejak, S., Stasiak-Różańska, L., Markowski, K., Lipińska, E. (2011). INCREASE THE ABILITY OF BIOSYNTHESIS OF DIHYDROXYACETONE BY GLUCONACETOBACTER XYLINUS BY UV MUTAGENESIS. Acta Sci. Pol. Biotechnol., 10(2), 17-24.
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