Acta Scientiarum Polonorum

Scientific paper founded in 2001 year by Polish agricultural universities

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Biotechnologia
(Biotechnologia) 10 (3) 2011
Title
GENETIC VARIABILITY BEETWEN SELECTED GENOTYPES OF PALEOENDEMIC CHINESE SPECIES – DAVIDIA INVOLUCRATA INTRODUCED TO WESTERN POMERANIA
Autor
Danuta Kulpa, Miłosz Smolik, Marianna Dobrochowska
Keywords
Davidiaceae, genetic diversity, ISSR-PCR
Abstract
The aim of conducted studies was to determine genetic and morphological variability of selected Davidia involucrata specimens from Western Pomerania and Berlin us- ing ISSR-PCR technique. The studies were carried out on the Davidia involucrata var. vilmoriniana growing in Germany in the Botanical Garden in Berlin-Dahlem and in Poland, in Pomerania: in the Dendrological Garden in Przelewice, Glinna and Central Cemetary in Szczecin. ISSR technique made it possible to determine genetic variability of the examined specimens. This was done by means of 6 out of 30 ISSR primers used in the experiment. Six primers (802, 807, 810, 819, 839, 840) generated in PCR reactions 64 amplicons, of which: 11 were monomorphic, 31 – polymorphic and 12 – genotype-specific. On average 1 primer generated 10 amplicons which ranged from 2550 to 270 bp.
Pages
27-36
Cite
Kulpa, D., Smolik, M., Dobrochowska, M. (2011). GENETIC VARIABILITY BEETWEN SELECTED GENOTYPES OF PALEOENDEMIC CHINESE SPECIES – DAVIDIA INVOLUCRATA INTRODUCED TO WESTERN POMERANIA. Acta Sci. Pol. Biotechnol., 10(3), 27-36.
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