Determination of the optimal types and concentrations of plant growth regulators as medium constituents is one of the most important factors of successful micropropagation. With the aim of optimization of in vitro multiplication of Clematis viticella and Clematis ‘Petit Faucon’ the effect of following cytokinins on growth and branching was studied: benzyladenine (BA), isopentenyl adenine (2iP), kinetin (KIN) and thidiazuron (TDZ). The obtained results show that KIN at concentration of 10 mg·dm^-3or 5 mg 2iP∙dm^-3 are the best for proliferation of Clematis viticella shoot tips while nodal parts produce more axillary shoots on the media with 2iP at concentration of 0.62 mg·dm^-3. Shoot tip explants of Clematis ‘Petit Faucon’ produce the most axillary shoots in the presence of 20 mg 2iP∙dm^-3 or 10 mg KIN∙dm^-3 and nodal ones branch best on the media with addition of 1.25 mg 2iP·dm^-3.

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