The aim of this study was to evaluate the range of variability in roots morphometric properties of selected varieties of barley. Three varieties of husky barley, Rastic, 43302 and 41267, and one variety of hulled barley, Bies, was tested. The roots were collected at the experimental field using core method. The roots were washed using a hydropneumatic elutriation system. Before scanning, any organic contaminations were manually removed. The images were analyzed using Aphelion, the software for image analysis and roots characteristics. The root length density (RLD), specific root length (SRL), mean diameter (MD) and dry matter (RDM) were calculated. The longest and the thinnest roost were determined for the 43302 variety. The 41267 was characterized by the lowest RLD value in all soil layers. The roots of Bies variety were the thickest but the shortest. However, the Rastic reach the highest value of RLD.

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