Studies with cultivation of Dactylis glomerata (cultivar Borna), Festuca pratensis (cultivar Ureus) and Lolium perenne (cultivar Inka), were carried out in the polyurethane pots, in 4 replications, on the experimental area of Department of Grassland and Green Area Creation. Eight seeds of one of the tested species were sown into each of the pots. After germination of grains, when the seedlings reached the 2-3 leaf stage, negative selection was carried out by removing 4 weakest plants. Then, four experimental factors in the form of the following fertilizer combinations were introduced: control – without fertilization, NPK – mineral fertilization, UG – microbiological fertilizer, UG + NPK – combined rates as for combinations NPK and UG. The experiment was carried out in 2008-2010. Detailed study included the following characteristics: aboveground biomass yield (g DM·pot^-1) and leaf greenness index (SPAD). All tested grass species reacted to fertilization applied in the experiment. However, significantly highest yield occurred on the objects with mineral fertilization combined with a microbiological fertilizer (on average 96.97 g DM·pot^-1). The largest amount of chlorophyll pigment occurred in plants cultivated on soil with a microbiological fertilizer and with a microbiological fertilizer together with mineral fertilization. The study showed usefulness of a microbiological fertilizer in the cultivation of Dactylis glomerata, Festuca pratensis and Lolium perenne. However, its highest effectiveness was observed in combination with mineral fertilization.

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