Most of known populations of microorganisms evolved adhesive abilities leading to a biofilm formation which is a unique microecosystem. This study is focused on dynamic of adhesion and biofilm formation in anaerobic conditions by the methanogenic consortium. Within this work the microscopic method enabling estimation of the number of the mathanogenic Archaea has been developed. Identification of methanogenes was based on autofluorescence of F₄₂₀ and F₄₃₀ coenzymes. Initially, the number of cells in methanogenic biofilm, was 4,4 x 10⁴ cm^-2 (at 6 h colonization), then increased to 1 x 10⁷ cm^-2 (on day 27 of colonization). Biofilm formation was faster on the plate section within the supernatant. The crucial point in biofilm formation was around 21 day, after which aggregates (microcolonies) were significantly increasing and fluorescent methanogens accumulated.

17-28