The abilities of Yarrowia lipolytica yeast to biodegradation of fatty substances in spent bleaching earth (SBE) were investigated. Yeasts inocula (of different number of propagation units) were added to SBE as free or aliginate immobilized (beads) biomass. The studied samples with SBE and yeasts were buried in soil and after winter the microbiological and chemical analyses were done as well as beads resistance and yeasts viability. The immobilized Y. lipolytica inoculum stimulated autochtonic microflora growth. The most important decrease of fatty substances in SBE was observed in presence of 50% of beads. Whereas, non depended on form and dose of yeasts was the composition of fatty acids in SBE samples, which was similar for different inocula but different from autonomous one. The effect of metabolic changes engendered by biomass suspension and immobilized cells was the decrease of contents of fatty acids of C6 – C8 and increase of C9, C16-C18. So, the inoculation of SBE with yeast could causes a specific bioconversion of fatty substances to long chain fatty acids. Noteworthy is the fact that bioremediaction was going on during winter period.

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