Solanaceae plants are a source of large number of valuable metabolites with multiple use both in vivo and in vitro conditions. Leaf explants obtained from N. tabacum ‘Samsun’ were cultivated on solidified MS medium supplemented with 3% (w/v) sucrose and enriched with different doses of extracts obtained from seedlings of the same plant cultivar or from N. tabacum ‘Havana’. The aim of experiments was to verify the effectiveness of such medium additives on callus tissue vitality, proliferation and its organogenetic response. It was ascertained a significant positive influence of all kinds of added extracts on increase of callus fresh weight and the organogenetic capability of tobacco cultures. However, the impact of seedlings devoid of roots Nicotiana extracts was significantly lower in both: stimulating of cell proliferation and shoot formation than extracts obtained from whole seedlings. The biological activity was the most effective in the case of genotype compatibility between the plant material from which the extract was derived with the object of biotest.

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