Four spring oat seeds new lines harvested between 1997 to 1999 years were characterized by basic chemical composition and biological activities in relation to agro-environmental factors such as fertilisation, precipitation and air temperature. Ash content, fat and reducing sugars as well as the level of extractable proteins, amylolytic and inhibitory activities tested against bovine pancreas trypsin and α-amylases of mammalian and insect origin were strongly affected by agro-environmental conditions which prevailed during the new oat lines growth. In three consecutive years of grain harvest the moisture and crude protein were also affected, however less by agro-environmental condition. The oat line CHD 1296 (registered as Hetman variety in 1999) showed quite stabile and high crude protein content over 13%, irrespective of different agro-environmental conditions during three consecutive years of growth.

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