In the years 2005-2009 in Szelejewo (Wielkopolskie voivodeship) a field experiment was carried out, aiming at the assessment of response of seed yield and autumn regrowth green forage of three cultivars of timothy: Prosna, Obra and Nowinka to growing in two row spacings: 24 and 36 cm. The research was conducted in two series including the establishment year and two years of production. It was indicated that in the first production year the cultivars Prosna and Obra gave similar yields, whereas in the second year Prosna gave a higher seed yield. The cultivar Nowinka was characterized by the lowest productivity in growing for seeds in both production years. In the first year, the use of row spacing of 24 cm had more favourable effect on forming fertile shoots and seed yield in comparison with row spacing 36 cm. In the second year, seed yield in both row spacings was similar. In the first production year the cultivar Prosna gave the highest yields of autumn regrowth green mass, and Obra the lowest.

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