The occurrence of fungi from the genus Trichoderma constitutes a serious problem in the culture of Agaricus bisporus leading to considerable losses. The aim of the investigations was to compare mutual interaction between some isolates of Trichoderma sp. and single-spore heterokaryotic cultures of A. bisporus on agar media and in cultivation conditions. The first stage of the study comprised assessment of growth for isolates of T. harzianum, T. agrressivum, T. atroviride and T. hamatum on PDA medium and growth of four heterokaryotic single-spore and strain Amycel 2600 A. bisporus cultures on peat-manure substrate. Moreover, the individual biotic effect (IEB) between isolates of Trichoderma and the tested A. bisporus cultures was also analysed. The second stage consisted in the evaluation of the volume of A. bisporus yield on the substrate, to which mycelia isolates of Trichoderma sp. were introduced. It was found that the analysed cultures and the cultivar strain of A. bisporus responded differently to the presence of Trichoderma sp. isolates both in terms of mycelium growth and the yield volume.

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