Parental lines are propagated in vitro in asparagus breeding. Additionally, in order to obtain super males which are needed for breeding all-male hybrids, anther culture is applied. Healthy and viable asparagus parental plants developed from in vitro culture should contain well formed storage roots. There are many factors affecting asparagus rooting. Only with the healthy explant materials, proper culture medium and growth conditions can asparagus plantlets be produced and later successfully grown in the greenhouse. In this article, obstacles and factors affecting asparagus rooting in vitro as well as trends in developing rooting technique are summarized.

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