Dihydroxyacetone (DHA) is ketotriose with reducing properties. It is used mainly in food industry (a sweetener, a dietary supplement, emulsifier, plasticizer), cosmetics (the active ingredient in self-tanning creams) and medicine (treatment of vitiligo desease, a component of biomaterials stopped bleeding). Currently, dihydroxyacetone is produced biotechnologically by incomplete oxidation of glycerol with acetic bacteria using Gluconobacter oxydans ATCC 621 strain. The enzyme catalyzing this reaction is PQQ – dependent glycerol dehydrogenase (GlyDH). The paper presents the physical and chemical characteristic of DHA, its application and receive. It also describes methods for improving the production of this compound, which have been developed over many years.

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