The aim of the present study was to compare the influence of the seed culture medium (YNB or mineral) and the sort of corn steep (liquor or powder) on the biosynthesis of pyruvic and α-ketoglutaric acids from crude glycerol by Yarrowia lipolytica A-10 strain. Batch cultures were carried out until the substrate exhaustion (100 g∙dm^-3 of glycerol) and lasted 68–118 h. Yeast produced from 30.4 to 35.8 g∙dm^-3 of pyruvic acid and from 14.4 to 17.9 g∙dm^-3 of α-ketoglutaric acid. Higher values of volumetric production rate of both acids were achieved when YNB was used as a seed culture medium. The yield of ketoglutaric acid production was similar in all culture variants. However, for pyruvic acid the highest value of this parameter (0,35 g∙g^-1) was obtained when mineral medium and corn steep liquor were used. The highest percentage of protein, about 38%, was determined in yeast biomass derived after the culture with mineral seed culture medium.

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