Twenty-four mutants in strain Yarrowia lipolytica Wratislavia K1 were obtained after mutagenesis induced by UV irradiation. The selection, on the basis of the growth ability on YNB medium containing erythritol, indicated nine strains with the size of colony surface similar to parent strain. In the 7-day shake-flasks experiment, in glycerol media, yeast produced from 10.3 to 16.6 g^.dm^-3 of erythritol and from 11.5 to 17.9 g^.dm^-3 of mannitol. For further studies in bioreactor cultures strains K1-UV20 and K1-UV21 were chosen. The highest concentration of erythritol was obtained by strain K1-UV21 on crude glycerol medium, where 78.7 g^.dm^-3 of erythritol was produced corresponding to 0.49 g·g^-1 yield and 0.68 g^.dm^-3h^-1 volumetric productivity. The analysis of philogenetic similarity of based on RAPD-PCR method showed high similarity of the mutant strain Wratislavia K1-UV21 to parental strains of Y. lipolytica.

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