Determination of the concentration and quality of DNA in the examined preparations is the first and fundamental act in studies of genetic material and is important in the further analysis. The result obtained has a decisive influence on the taking of further research. The aim of this study was to compare the quantity and quality (purity in terms of protein contamination, reagents for the isolation of cell particles and pollution) of DNA isolated from fresh chicken blood and kept using two commercial kits. The research material was the blood drawn from a vein v. basilica, fresh and stored for 24 months (frozen) at -20°C. Fresh and stored blood came from 10 individuals Polbar breed chickens. Stored blood came from 10 other individuals of the same race. Isolation of DNA was carried out based on two different sets of genomic DNA isolation: Genomic Midi AX, A & A Biotechnology and QIAamp DNA Blood Mini Kit. A statistically significant difference in concentration of different sets of DNA isolated isolation in fresh and kept blood. A better kit for isolation of DNA from chicken blood was putty Genomic Midi AX, a higher concentration of DNA obtained from fresh blood.

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