Yarrowia lipolytica are an nonconventional microorganisms able to assimilate organic compounds and produce extracellular enzymes. The current trend of their use is bioconversion of secondary metabolites (mainly citric acid) and aroma compounds (γ-lacton) and obtaining oleic cells used in fodder production. The microorganisms are characterized by high proteoand lipolytic activity. They are able to synthesize and secret extracellular proteases: serine (AEP) and aspartyl (AXP) and lipases (LIP1, LIP2 i LIP3). The expression of those enzymes is pH dependent and influenced also by the yeast growth phase and availability of carbon, nitrogen and sulfur sources. The recent years research points out many different possibilities of Y. lipolytica enzymes utilization. The easy way of their isolation combined with the use of food industry waste-products makes them an attractive and competitive product in comparison to commercially avaliable enzymes.

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