In case of sulphur shortage in the soil that element has a significant impact on yields of plants and their quality. The objective of the work was the impact of sulphur fertilization on content of Mg and Ca in the dry-mass and in yield of dry-mass of potato tuber. Experience in the field of potato head was in the years 2004–2006 by applying different kinds of sulphur (elemental and K₂SO₄) and rate (0, 25 and 50 kg·ha^-1). The content of Mg and Ca in the dry mass and Mg content in yield of dry mass of potato tuber was significantly determined by S fertilization. The highest content of Mg and Ca was found when using 25 kg S∙ha^-1 in elemental kind and 50 kg S∙ha^-1 in elemental and sulphur kind. Mg content in yield of dry mass of tubers increased S-elemental fertilization regardless of the rate, while this parameter of Ca no depended on S-fertilization. Sulphur fertilization in sulphate kind increased content S-SO₄ in the soil, while S-elemental fertilization in rate 50 kg∙ha^-1 decreased pH value of soil. Negatively correlation was between pH value of soil and Mg content in dry-mass of tuber. Negatively correlation was also between pH value of soil and Ca content in yield of dry mass of potato tubers.

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