Allium neapolitanum is a valuable species of snow-white flowers, which is suitable for cultivation in flowerbeds, rock gardens as well as in containers. Whole buds of Allium neapolitanum were excised from bulbs in the beginning of October and then they were cultured on Murashige and Skoog (MS) medium containing 2 mg BA·dm^-3 and 0.1 mg NAA·dm^-3 for shoot initiation. After several passages on the same medium for shoot multiplication, bases of shoots were placed for 2 subcultures on MS medium supplemented with BA or 2-iP in concentration of 2 or 5 mg·dm^-3 separately or in combination with NAA in concentration of 0.1 or 1 mg·dm^-3 to obtain multiplication. MS medium without growth regulators was used as a control. The best results were obtained on the medium supplemented with 5 mg BA·dm^-3 and 0.1 mg NAA·dm^-3. On average 5.7 shoots regenerated from 1 shoot base during 12 weeks. Three types of auxins, IAA, IBA and NAA in concentration of 0.5 mg·dm^-3, were used for rooting. It was observed that NAA enhanced root formation but reduced roots length. The best quality rooted shoots were obtained on medium supplemented with 0.5 mg·dm^-3 IAA. The survival rate of the plantlets under ex vitro condition was 70% after 4 weeks.

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