The usefulness of the enzyme preparation Fyre-Zyme (trade name) for purification of hydrocarbon-contaminated soil was investigated under laboratory conditions. Parallelly, its influence on the dynamics of soil microflora has been tested. Fyre-Zyme, which does not currently have a certificate in Poland, was the most effective during the first 6 hours of experiment. At this time, in comparison to control soil (without Fyre Zyme), the content of petroleum substance decreased by 29% and after 60 days by 52%. Fyre-Zyme preparation assisted the degradation of n-aliphatic hydrocarbons and polycyclic aromatic hydrocarbons (PAHs) but did not affect in loss of mono-aromatic hydrocarbons (BTEX). The changes of soil biotic relations were observed at presence of Fyre-Zyme – decrease of the number eukaryotic microflora and increase of prokaryotic microflora during 60 days of process. However, the enzyme preparation had no influence on the number of hexadecane degrading microorganisms.

5-18