Acta Scientiarum Polonorum

Scientific paper founded in 2001 year by Polish agricultural universities

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Biotechnologia
(Biotechnologia) 12 (1) 2013
Title
YEASTS MICROFLORA OF NATURALLY FERMENTED VEGETABLES
Autor
Zbigniew Lazar, Michał Piegza, Ewa Walczak, Wojciech Barszczewski, Małgorzata Robak
Keywords
yeast, RAPD, PCR, RLFP, pickles
Abstract
This research aims at determining which yeast types can be encountered in the following home products: pickled cucumbers, sauerkraut and mixed pickled vegetables. Seventy-five strains of yeast have been isolated from the pickles selected for the research. These strains have been identified on the basis of API tests (bioMerieux), amplification of the selected regions of rDNA genes (NS3-ITS4) and restrictive analysis, as well as genome scanning, performed in order to detect microsatellite sequences. Yeast Saccharomyces cerevisiae (38.7% isolates) and Yarrowia lipolytica (25.6% isolates) have been determined as a dominant microflora for the pickles. There have been also isolated strains belonging to the following types: Pichia etchellsii, P. ohmerii, Candida holmii, C. pelliculosa and Shizosaccharomyces japonicus. With the use of API 32C, some isolates have been identified merely in accordance to their type, and due to a limited data base of model strains, not all the isolates have been possible to be classified on the level on their type with the support of molecular techniques. The obtained results of the research allowed to supply the database with new yeast strains (strains of different restrictive profiles), which should consequently facilitate conducting the identifying works.
Pages
19-36
Cite
Lazar, Z., Piegza, M., Walczak, E., Barszczewski, W., Robak, M. (2013). YEASTS MICROFLORA OF NATURALLY FERMENTED VEGETABLES. Acta Sci. Pol. Biotechnol., 12(1), 19-36.
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