Determining a proper level of nitrogen fertilization for new cultivars of winter wheat is of great importance with regard to this component’s effect on the grain yield height and its quality. The aim of the study was determination of the effect of doses and methods of nitrogen fertilization (soil and foliar application) on the grain yield, yield components, total protein content and chosen macro- and microelements in winter wheat grain. The field experiment with winter wheat cv. Rywalka, was carried out in seasons 2008/2009, 2009/2010 and 2010/2011 on a farm located in the south-east Poland. The experiment was set up with the use of a randomized block design in four replications. The studied factor were diverse doses of nitrogen top-dressing from 40 to 120 kg·ha^-¹, soil and foliar application, compared to the control without nitrogen fertilization. The field experiment was set up on a brown soil, bonitation class IIIa. Within the years of research, the soil indicated a variable content of available mineral components. Soil reaction in KCL was from 5.9 to 6.7. Weather conditions during wheat’s growing season were variable in the years of research. The growing season 2009/2010 abounded in rainfall, of which the total amount was 950 mm from October to August. Growing seasons 2008/2009 and 2010/2011 were characterized by a favorable course of weather conditions. Winter wheat yield cv. Rywalka was diversified within the years of research, and depended on the top-dressing with nitrogen. The highest yield was obtained after an application of nitrogen at a dose of 120 kg·ha^-¹. Top-dressing with nitrogen affected some yield components of winter wheat. Plants fertilized with 120 N kg·ha^-¹ were characterized by a significantly higher spike density. Along with higher doses of nitrogen fertilization, also the total protein content increased in the grain. It was significantly higher after an application of 120 kg·ha^-¹ as well as 84 kg·ha^-¹, than in the grain of plants which were not fertilized. Top-dressing with nitrogen modified the content of magnesium and calcium, however, it had no significant effect on the accumulation of phosphorus and potassium in wheat grain. Under the effect of top-dressing with nitrogen, the content of manganese and zinc in the grain underwent oscillations, while the content of iron and copper was not significantly diversified.

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