The field experiment was carried out at the Mochełek Experiment Station of the Faculty of Agriculture, University of Technology and Agriculture over 1984-1994. Its aim was to define an effect of nitrogen fertilisation on the amino acid composition in winter barley grain protein. Four levels of nitrogen fertilisation: 0, 60, 120 and 180 kg N·ha^-1 were investigated. Applying 120 kg N·ha^-1 results in a lowered content of most amino acids, the exogenous ones especially, as compared with the control. The total sum of amino acids in the winter barley grain protein decreased from 893.2 g·kg^-1 in the control to 846.1 g·kg^-1 in the object treated with 180 kg N·ha^-1.

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