The object of the study was to determine the effect of the pretreatment of Miscanthus giganteus and straw rape with a 15% solution of ammonia on the availability of polysaccharides in substrates for hydrolysis. Its effectiveness was assessed on the basis of the concentration of reducing sugars released during enzymatic hydrolysis and its efficiency calculated in relation to the total polysaccharides available in materials. Carrying out the pretreatment under conditions of 80°C/6 h resulted in an increase in released sugars concentration by 50% (Miscanthus) and 18% (straw), with regard to the hydrolysis of the materials pretreated under conditions of 20°C/24 h, at the same hydrolysis time. Regardless of the pretreatment variant a higher degree of delignification was reported for Miscanthus than rape straw.

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