The parameters of pyruvic acid production by Yarrowia lipolytica A-10 strain from pure and crude glycerol at different C:N ratio (26.8 and 17.9) were compared. The highest pyruvic acid concentration was achieved in culture with C:N ratio 26.8, using crude glycerol as a substrate. After 92 hours of cultivation yeast produced 58.2 g∙dm^-3 of pyruvic acid with yield 0.39 g∙g^-1 and volumetric production rate 0.63 g∙dm^-3∙h^-1. Biomass from this process was separated from cultivation medium and determined for quantity of protein and intracellular fat. Cells of Yarrowia lipolytica A-10 were composed of 43.9% of protein and 10.42% of fat, in which unsaturated acids represented the majority of total fatty acids (linoleic acid, 18:2 – 33.94% and oleic acid, 18:1 – 30.98%).

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