On the basis of the conducted research and analyses, in the years 1972, 1992, and 2010, changes of the chosen chemical properties of soil were evaluated, where five cereal species were grown in two environmentally correct crop rotations and in single-species monocultures. In 1972, set as the starting year for change evaluation, the content of organic carbon and assimilable phosphorus were usually higher in the soil of cereals grown in crop rotation than in monoculture. Soil pH and the content of other assimilable microelement forms did not demonstrate this type of one-way tendencies. Depending on the cereal species, their higher content was noted in either crop rotation soil or in monoculture. In 1992, after 20 years of manure-free plant cultivation, a decrease in organic carbon content was found, usually higher in monocultures than crop rotations. Assimilable calcium content decreased, whereas soil pH and the contents of other assimilable forms of macroelements increased. Manure fertilization, applied from 1992, attributed to the increase in the content of organic carbon in the soil. After 18 years of its application, organic carbon content almost on all the plots was higher than in the initial year 1972. However, soil pH decreased, as did, with few exceptions, calcium content. The content of other macroelements, depending on the cereal species and cultivation post, has increased or decreased.

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