Biosorption process was used for obtaining of mineral mixtures for sheep enriched with zinc, manganese, cooper and iron ions. VFA (volatile fatty acids) profil in rumen chyme consists of etan acid, propan acid, and butan acid in proportion 11:2.2:1, what indicated normal physiological process. There were slight changes in reciprocal influence of these acids after addition of substrates in 4th and 8th hour of fermentation. The results analysis proved slight decrease of ethanoic acid level in 24th hour of fermentation in groups with mixture enriched with supplements after biosorption process. Furthermore others indicators of fermentation, measured based on VFA level revealed small decrease of ethanoic acid concentration. Production of isobutanoic acid and isopentanoic acid in experimental groups as well as in control group at 8th hour of fermentation decreased. Increase of methane production and NGR index were observed in samples enriched with mineral compounds. Conducted experiments showed, that mineral supplements for sheep enriched in biosorption process have no negative influence on rumen fermentation products. The maintenance production of VFA in 4th and 8th hours of fermentation revealed advantageous influence of using substrates in activity of symbiotic microorganisms participating in rumen fermentation.

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