The aim of the study was the evaluation of the effect of diversified nitrogen fertilization: in-soil, foliar, and foliar in combination with multi-component fertilizer Ekolist, on the content and composition of protein of spring triticale grain cultivar Milewo. Field experiment was carried out at the Didactic-Experimental Centre in Tomaszkowo, which is part of the University of Warmia and Mazury in Olsztyn. Total protein content, as well as the contents of the particular protein fractions in spring triticale grain cultivar Milewo were affected by both the application of foliar additional feeding with Ekolist and mineral nitrogen dose. Nitrogen application at the dose of 80 kg·ha^-1 with Ekolist, in comparison with the same dose but without the multi-component fertilizer, attributed to the increase in protein amount by 0.72 g per 100 g of grain. The highest proportion was made of storage proteins with the advantage of gliadins over glutenins. Fertilization variants applied in the experiment resulted in a high ratio of gliadins to glutenins. Moreover, significant effect of the total nitrogen dose 120 kg·ha^-1 was confirmed in comparison with 80 kg·ha^-1 on the increase in the concentration of ωand α/β gliadins . Cultivation year affected the proportion of γgliadins and HMW and LMW glutenins in the grain. Increase in nitrogen fertilization by 40 kg·ha^-1 before sowing as ammonium nitrate contributed to the increase in ω gliadins and HMW glutenins. Additional application of Ekolist fertilization (variant with the dose of 80 kg·ha-1) caused an increase in γ gliadins and LMW glutenins.

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